Hyaluronidase activity in human semen correlation with semen parameters and fertilization in vitro.

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Published by National Library of Canada in Ottawa .

Written in English

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Thesis (M.Sc.) -- University of Toronto, 1994.

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SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
Pagination2 microfiches : negative. --
ID Numbers
Open LibraryOL17041826M
ISBN 100315963484

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Assited reproductive technology Hyaluronidase activity in human semen: correlation with fertilization in vitro* †* Supported by Medical Research Council of Canada, Ottawa, Ontario, Canada.

†Presented in part at the Canadian Fertility and Andrology Society, St. Andrew’s by the Sea, New Brunswick, Canada, September 7 to 9, Cited by: Hyaluronidase activity was expressed as the mean of the duplicate measurement of the ring diameter. The diameter of the well was subtracted to give a ring size related to the depth of hydrolysis.

Multiple regression analysis was used to assess the correla­ tions between hyaluronidase activity and the sperm. Hyaluronidase is an acrosomal enzyme which participates in the dissolution of the cumulus oophorus matrix, containing hyaluronic acid, and is essential for the fertilization process.

In this study hyaluronidase activity was determined in two fractions of split normozoospermic and oligozoospermic human by: 4. The following results were obtained by this method in clinical practice. A significant correlation was found between hyaluronidase activity and sperm concentration in Hyaluronidase activity in human semen book infertility.

Hyaluronidase activity increased as the concentration of sperm increased, and the least hyaluronidase activity was determined at < 10 × 10 10 × / by:   Hyaluronidase activity was examined in oligospermic and normospermic human semen specimens promptly after liquefaction, after repeated cycles of washings with a solution containing M sucrose and M NaCl, and after freezing and thawing.

We assessed enzyme activity by measuring areas of the digestion of substrate (hyaluronate).Cited by: 1. The human sperm protein PH has hyaluronidase activity Michael Gmachl, Sandrine Sagan**, Sigrid Ketter, Giinther Kreil* Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstra A Salzburg, Austria Received 22 November The PH protein present on the membrane of guinea pig sperm was characterized using a.

Hyaluronidase activity in split human semen. Savion M, Singer R, Sagiv M, Segenreich E, Servadio C. Int J Fertil, 31(3), 01 Jul Cited by: 0 articles | PMID: [Hyaluronidase activity of human spermatozoa] Di Bella S, Panazzolo A.

The human sperm protein PH has hyaluronidase activity. Hyaluronidase activity in human semen book Gmachl M., Sagan S., Ketter S., Kreil G. The PH protein present on the membrane of guinea pig sperm was characterized using a monoclonal antibody [() J. Cell Biol. ]. We have isolated the cDNA encoding the human PH protein from a testis library.

This current model lacks a well- supported explanation for how sperm penetrate the cumulus layer. We report that the sperm protein PH has a hyaluronidase activity and is present on the plasma membrane of mouse and human sperm. Brief treatment with purified, recombinant PH can release all the cumulus cells surrounding mouse eggs.

Hyaluronidase activity was measured by a colorimetric assay [29]. One unit of enzyme activity was defined in terms of the amount required to digest 50 % of the substrate (hyaluronic acid, Sigma) under defined conditions (30 min of incubation, 37 "C, pg substrate ml-', M sodium acetate buffer containing M NaCl, pH ).

This current model lacks a well-supported explanation for how sperm penetrate the cumulus layer. We report that the sperm protein PH has a hyaluronidase activity and is present on the plasma membrane of mouse and human sperm. Brief treatment with purified, recombinant PH can release all the cumulus cells surrounding mouse eggs.

hyaluronidase obtained from Column IV which showed one major component (Fig. 3) on 7%75 gel at pH One major TABLE I Summary of purijkation of sperm hyaluronidase from bull sperm Procedure Total Specific Total Relative protein activity activity activity G= II nilP/m prorern 13 16 50 1, 2, 8 Yield.

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Reese KL, Aravindan RG, Griffiths GS, Shao M, Wang Y, Galileo DS, Atmuri V, Triggs-Raine BL, Martin-DeLeon PA () Acidic hyaluronidase activity is present in mouse sperm and is reduced in the absence of SPAM1: evidence for a role for hyaluronidase 3 in mouse and human sperm.

Mol Reprod Dev 77(9)– CrossRef PubMed PubMedCentral Google. Keywords: Cumulase®, human, hyaluronidase, ICSI, oocyte, recombinant Intracytoplasmic sperm injection (ICSI), involving the injection of a single sperm into the cytoplasm of an oocyte using a fine glass needle, was first successfully used in and has subsequently become the widely accepted treatment for couples.

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida.

The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

E-Mail Address. Password. Forgotten Password. Remember Me. changes, which control hyaluronidase activity at varying pH are unknown.

Human serum hyaluronidase 1 (HYAL1) is active solely below pH Here we report the design of a HYAL1 variant, that degrades hyaluronan up to pH Besides highly conserved residues in close proximity of the active site of most hyaluronidases, we identified a bulky loop. Sperm hyaluronidase has long been believed to participate in sperm penetration through the cumulus matrix.

However, our previous works using male mice lacking either one of two sperm hyaluronidases, SPAM1 and HYAL5, conclusively showed that neither of these hyaluronidases is essential for fertilization. In this study, we examined whether the hyaluronan-degrading activity of mouse epididymal.

The sperm plasma membrane protein PH has a hyaluronidase activity that enables acrosome-intact sperm to pass through the cumulus cell layer of the egg.

The specific activity of the 57 kDa and 75–79 kDa coagulum proteins, and that of serum albumin against Sigma bovine testicular hyaluronidase (type IV), were 11and units per mg protein respectively. It was speculated that human seminal coagulum proteins may have an activation effect on spermatozoal hyaluronidase activity in vivo.

Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant.

Reproductive Toxicology. When a capacitated sperm reaches the ovum, it is able to penetrate this layer with the assistance of hyaluronidase enzymes present on the surface of the sperm. Once this occurs, the sperm is capable of binding with the zona pellucida, and the acrosome reaction can occur.

Thus hylauronidase helps the sperm to reach ovum plasma membrane. Recently, it was reported that SPAM1-deficient mice are fertile and spermatozoa derived from mutant mice still exhibit hyaluronidase activity [Baba, Kashiwabara, Honda, Yamagata, Wu, Ikawa, Okabe. A new affinity method for purification of bovine testicular hyaluronidase enzyme and an investigation of the effects of some compounds on this enzyme.

Journal of Enzyme Inhibition and Medicinal Chemistry30 (4), Sperm motility and hyaluronidase activity were not adversely affected by glycation.

Together, the observed detrimental effects of the increased levels of AGE on DNA integrity, without an effect on motility and hyaluronidase activity, suggest that sperm may retain some fertilizing capacity under these adverse conditions. Composition of Human Semen Composition of Human Semen.

Semen is the grayish white bodily fluid which is emitted from the urethra during ejaculation. Semen contains sperms which can fertilize the female ova to produce new life. The average volume of semen in an ejaculation is about ml. Hyal-1, the best-characterized somatic hyaluronidase (3, 4, 5), product of one of the six hyaluronidase-like sequences in the human genome, is an acid-active enzyme with an optimum at pH The sperm-specific PH (7) has apparently two pH optima, pH andresulting possibly from two forms of the enzyme, membrane-bound and soluble.

The Human Hyaluronidase PH (SPAM1, Hyal-PH20) ELISA kit is to be used to detect and quantify protein levels of endogenous SPAM1. It recognizes human SPAM1 within the range of pg/mL. A human recombinant hyaluronidase kit, HyQvia, was approved for use in the European Union in Mayand in the United States in September [23] [24] It is a dual vial unit with one vial of immune globulin infusion 10% (human) and one vial of recombinant human hyaluronidase.

[25]. The Human Reproductive System This scanning electron microscope image enlarges human sperm cells on a human egg cell thousands of times. You will read in this chapter that after one sperm cell enters an egg cell, other sperm cells cannot.-V i ViÊÊ-V i ViÊÊ+PVSOBM Imagine that you are a sperm cell entering an egg cell.

Therefore, all mammalian spermatozoa bear hyaluronidase activity. A protein, first of all characterized with the aid of a monoclonal antibody against posterior head determinants and thus named PH (33), could later be shown to exhibit this particular activity (34).

Unfortunately, PH was also named sperm adhesion molecule 1 (SPAM1) (35). Lin, Y, Mahan, K, Lathrop, W F, Myles, D G, Primakoff, P: A hyaluronidase activity of the sperm plasma membrane protein PH enables sperm to penetrate the cumulus cell-layer surrounding the egg.

Cell Biol.  Spermiation: Release of mature sperm into the lumen of seminiferous tubule. Question What do you mean by ‘Sperm lysin’. Mention its function. Answer: Sperm lysin is a proteolytic enzyme secreted in the acrosome of sperm.

It helps to penetrate the ovum during fertilization. It is also called as hyaluronidase. Question Human Reproduction - Live Session - 18 Nov Contact Number: / The human sperm pellets, containing 50–75 × 10 6 cells, from both treated and control groups were washed twice with 10 mmol/l phosphate buffer, pHand were separated at g for 10 min at 0–4°C.

The washed sperm were then extracted with 1 ml. The most widely conserved mammalian sperm antigen is sperm adhesion molecule 1, SPAM1/PH, which is also the major testicular hyaluronidase. This multifunctional glycosyl phosphatidylinositol (GPI)-linked protein plays several roles in fertilization and is encoded by a gene that resides among hyaluronidase family members in a cluster on human.

This family of enzymes has hyaluronidase activity which hydrolyses the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. Sperm hyaluronidase is active at neutral and acidic pHs which results from different active sites in the hyaluronidase domain at the N-terminus of the protein.

Effects of Iridoids on Lipoxygenase and Hyaluronidase Activities and Their Activation by β-Glucosidase in the Presence of Amino Acids Biological & Pharmaceutical Bulletin, Vol.

26, No. 3 Inhibitory activity of brown algal phlorotannins against hyaluronidase. كتاب An Investigation of the Effects of Ivermectin on Blood Serum,Semen Hyaluronidase Activities and Spermatological Characteristics in Sheep متاح للتحميل pdf بحجم 88 KB بتحميل مباشر دون اعادة توجيه لمواقع أخرى، حمل An Investigation of the Effects of Ivermectin on Blood Serum,Semen.

This study evaluated the effects of γ radiation on spermatozoa based on the morphological characteristics of sperm in the caudal epididymis of SWR/J mice.

In this study, various abnormal sperm shapes (amorphous heads, hook-less, mid-piece defect, pinheads, coiled tails, and tail-less) were observed after exposure to γirradiation (2 or 4 Gy)and after treatment with aspirin (ASA) .Semen is a thick, nutritious liquid, rich in vitamins (such as C, B12), amino acids, lipids, monosaccharides (fructose), et cetera.

Can semen's ability to fulfill the needs of human organism provide enough essential nutrients to keep the body alive and functional? And if so, would it be a healthy life?Rituximab and hyaluronidase human is a form of rituximab that is given as a subcutaneous injection.

This form can be given in less time than rituximab, which is given as an infusion. The subcutaneous form is used only after patients have received one full dose of rituximab by infusion.

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